人胰腺癌細胞PANC-1

細胞名稱

人胰腺癌細胞PANC-1                  

貨物編碼

BFN60700123

產品規格

T25培養瓶x1

1.5ml凍存管x2

細胞數量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研用途       1類

培養體系

DMEM高糖培養基（Hyclone）+10%胎牛血清（Gibco）+1%雙抗（Hyclone）

培養溫度

37℃

二氧化碳濃度

5%

簡介

人胰腺癌細胞PANC-1源自于胰腺癌導管細胞，其倍增時間為52小時。染色體研究表明，該細胞染色體眾數為63，包括3個*標記的染色體和1個小環狀染色體。該細胞的生長可被1unit/ml的左旋天冬酰胺酶抑制；能在軟瓊脂上生長；能在裸鼠上成瘤。 該細胞引種自ATCC CRL-1469

注釋

Part of: AKT genetic alteration cell panel (ATCC TCP-1029).
Part of: Cancer Cell Line Encyclopedia (CCLE) project.
Part of: ENCODE project common cell types; tier 3.
Part of: KuDOS 95 cell line panel.
Part of: MD Anderson Cell Lines Project.
Doubling time: 25.83 +- 2.03 hours (PubMed=25394408); 21 + 1.6 hours (PubMed=21515691); 52 hours (CLS); ~42 hours (DSMZ); ~32 hours (PBCF); 15.02 hours
Karyotypic information: Has lost chromosome Y.
Omics: Array-based CGH.
Omics: Deep exome analysis.
Omics: Deep proteome analysis.
Omics: Deep quantitative proteome analysis.
Omics: Deep RNAseq analysis.
Omics: DNA methylation analysis.
Omics: H3K4me3 ChIP-seq epigenome analysis.
Omics: Metabolome analysis.
Omics: Protein expression by reverse-phase protein arrays.
Omics: Proteome analysis by 2D-DE/MS.
Omics: SNP array analysis.
Omics: Transcriptome analysis.
Caution: Additional TP53 mutation in c.815T>C indicated incorrectly in PubMed=1630814.

STR信息

參考文獻

PubMed=31037374; DOI=10.1007/s00216-019-01814-1
Lagies S., Schlimpert M., Braun L.M., Kather M., Plagge J., Erbes T., Wittel U.A., Kammerer B.
Unraveling altered RNA metabolism in pancreatic cancer cells by liquid-chromatography coupling to ion mobility mass spectrometry.
Anal. Bioanal. Chem. 411:6319-6328(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402(2020)

PubMed=32058723; DOI=10.1021/acs.jproteome.9b00840
Tan Z., Zhu J., Stemmer P.M., Sun L., Yang Z., Schultz K., Gaffrey M.J., Cesnik A.J., Yi X., Hao X.-H., Shortreed M.R., Shi T., Lubman D.M.
Comprehensive detection of single amino acid variants and evaluation of their deleterious potential in a PANC-1 cell line.
J. Proteome Res. 19:0-0(2020)